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Top/fop flash reporter assay

Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay was used to analyze the activity of Wnt/β-catenin signaling pathway. The vector pRL-SV40 was served as the internal reference. Cells in the control groups were co-transfected with pRL-SV40 and TOP/FOP flash (Promega). Cells in NC groups were co-transfected with pRL-SV40, TOP/FOP flash and si … Web6. apr 2024 · The TOP/FOP-FLASH assay showed that the canonical Wnt activities were reduced in HONE1 and NPC43 cells, when THY1 was over expressed (Figure 2A). ... Luciferase reporter HONE1 cells were injected into nude mice via tail vein, followed by treatment with 10 mg/kg of KX2-391 or solvent control at a frequency of twice per week …

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Web11. apr 2024 · 1.5 × 10 4 SW620 or Difi cells were plated in 24-well plates, then co-transfected with the firefly luciferase reporter plasmid TOP-FLASH or FOP-FLASH and the Renilla luciferase vector phRG-TK at 1 µg/ml (kindly provided by Dr P. Blache, IRCM, Montpellier, France) and incubated with oxaliplatin (5 µg/ml). 72 h post-transfection, cells … Web17. júl 2024 · TOP/FOP-flash reporter assays. Cells were seeded in a 96-well plate, and the TOP/FOP-Flash (Genechem) was co-transfected into cells along with LINC01278 siRNA or overexpression plasmid. Luciferase activity was measured using the Promega Dual-Luciferase Reporter Assay System at 24 h after transfection. The luciferase activity was … microchip cat food bowls https://nunormfacemask.com

How to detect and activate Wnt signaling The Wnt …

WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold … Web86. Millipore luciferase top flash. Luciferase Top Flash, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … Web25. okt 2024 · レポーター遺伝子アッセイは、目的としている遺伝子とレポーター遺伝子を組み替えることによってどれぐらいの発現量があるのか調べる方法です。. レポーターというのは簡単に言うと、量を測定するのが簡便なタンパク質のことです。. よくある例で言え … microchip chandler address

How to detect and activate Wnt signaling The Wnt …

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Top/fop flash reporter assay

Top Flash Promega Bioz

WebPlasmid TOP-GFP from Dr. Ramesh Shivdasani's lab contains the insert 7xTOP-GFP and is published in Cancer Res. 2012 Feb 8. This plasmid is available through Addgene. ... We inserted the 7xTCF/LEF promoter cassette from the M50 Super-TOP flash plasmid (Randall Moon) into the pRRLSIN.cPPT.PGK-GFP.WPRE vector (Didier Trono), replacing the PGK ... Web31. máj 2024 · In vitro proliferation, migration and invasion assays, and an in vivo nude mice model were utilized to assess the important role of desmosomal genes on SOX30-induced tumor suppression. A WB assay and TOP/FOP flash reporter assay was used to investigate the downstream pathway regulated by the SOX30-desmosomal gene axis.

Top/fop flash reporter assay

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WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and … WebLuciferase reporter assays were performed by transfecting TOP/FOP Flash (A), Hes1 (B) or Hey1 (C) luciferase reporter in the cells treated with or without rapamycin (Rapa, 200nM, Sigma), an ...

Web10. jan 2024 · TOP/FOP-Flash reporter assay showed that CDX2 knockdown or CDX2 overexpression significantly increased or decreased Wnt signaling activity. Western blot … Web12. júl 2024 · The Dual-Luciferase Reporter Assay (Promega) was applied according to the manufacturer’s instructions. For the TOP/FOP-Flash assay, TOP/FOP-Flash (Genechem) was co-transfected into cells along with LUCAT1 silence or overexpression vector, miR-5582-5p mimic or inhibitor, and/or the miRNA control.

Web9. feb 2024 · TOP/FOP flash reporter assay HCT-116 cells were seeded in a 24-well cell culture plate and co-transfected with the pRL-TK plasmid (10 ng/well) and either TOP flash plasmid or FOP flash plasmid (200 ng/well). At 36–48 h later, the cells were analyzed using Dual-Luciferase Reporter Assay System (Promega, E1910) to assess the luminescence … Web10. jan 2024 · TOP/FOP-Flash reporter assay showed that CDX2 knockdown or CDX2 overexpression significantly increased or decreased Wnt signaling activity. Western blot assay showed that downstream targets of Wnt signaling, including β-catenin, cyclin D1 and c-myc, were up-regulated or down-regulated in CDX2-knockdown or CDX2-overexpressing …

Web• Established the use of the TOP/FOP FLASH Luciferase Assay in Dr. Wei’s lab. • Used TOP/FOP FLASH Luciferase Assay to monitor levels of canonical Wnt-activated transcription as a proxy for ...

WebTop Fop Flash Luciferase Reporter Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … microchip change of ownershipWeb背景技术微小RNA（miRNA）在肿瘤的发生和发展中起着至关重要的作用。以前，我们表明miR-504被下调并抑制胶质母细胞瘤（GBM）中的肿瘤增殖。然而，miR-504在GBM间充质（ME）过渡中的调控及其相关机制仍不清楚。方法转录组和临床数据来自癌症基因组图谱（TCGA）数据库。 the open university login #microchip chandler hiringWeb1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点 … microchip change of ownership formWebI have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and FOP, I couldn't see a high TOP/FOP ratio. microchip chandler jobsWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... microchip change of ownership vicWeb5. dec 2024 · TOP-Flash contained a wild type LEF/TCF-binding site, while FOP-Flash contained a mutant LEF/TCF-binding site. HCT116 cells were transfected with reporter … microchip chandler