Phis2 snapgene

WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... WebbpHIS2-hsSTAM1 (1-143) Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Partial (1) Partial Sequences from Addgene (1) Addgene has …

Addgene: pHIS2-hsSTAM1 (1-143) Sequences

Webb26 okt. 2024 · Rapidly Simulate a Restriction Digest. To simulate a restriction digest from a DNA sequence window, select one or more enzyme sites, then click Tools → Simulate Agarose Gel. The resulting digest will appear in the next available lane in the Agarose Gel Simulation dialog. Repeat this step to populate other lanes. WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … ts type string is not assignable to type https://nunormfacemask.com

Characterization of GALA (α-galactosidase) gene family and …

Webb9 juni 2014 · The results showed that only the positive control and the co-transformants of pGAD-Zmhdz10 and pHIS2-cis could grow on the SD/Trp–/Leu–/His– medium containing 40 mM 3-aminotriazole (3-AT) whereas the negative control did not grow (Fig. 3B). We also constructed a pHIS2-mcis vector, and co-transformed it with the pGAD-Zmhdz10 … WebbSee Command Line: Installation and Activation. macOS: The macOS installer is an Apple Disk Image file (.dmg). In Finder, open the disk image and drag the SnapGene application … Webb1 apr. 2024 · The enzyme α-galactosidase (GALA) is a glycoprotein that hydrolyzes molecules found in complex sugars. α-galactosidase is also widely distributed in different organs of high plants, including seeds, leaves, and fruits ( Fujiki et al., 2001, Soh et al., 2006, Zhao et al., 2006 ). ph levels and co2

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Phis2 snapgene

Yeast Reporter Vector Phis2 1 Bd TaKaRa Bioz

Webb24 aug. 2024 · The promoter of each gene was cloned and inserted into the pHis2.1 vector to generate reporter constructs. TcMYC2 was fused to the GAL4 activation domain to generate the effector construct pGADT7- TcMYC2 ( Fig. 4a ). The reporter and effector constructs were co-transformed into yeast cells. WebbpUC19 Vector. pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).

Phis2 snapgene

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Webb载体介绍. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a multiple ... Webb5 feb. 2024 · The growth performance of yeast strain Y187 containing the two constructs was observed on SD/-Leu/-Trp and SD/-Leu/-Trp/-His medium in the presence of 30 mM 3-AT. pGAD-53 was co-transformed with pHIS2-P53 as a positive control and pGADT7-CsATAF1 was co-transformed with pHIS2-P53 as a negative control. The experiment …

WebbSnapGene Academy WebbSnapGene Viewer. Free software that allows you to create, browse, and share richly annotated sequence files. Download now. SnapGene. The easiest way to plan, visualize …

http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf WebbSnapGene provides the easiest and most secure way to plan, visualize, and document your molecular biology procedures. That’s why scientists at leading institutions and companies around the globe ...

Webb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。. 方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。. 材料：. 1．启动子克隆：pHIS2-A。. 根据实验需求进行扩增并酶切构建载体，两端的EcoRI和. SacI酶切位点，序列如下 ...

Webb6 feb. 2024 · The chloroplast comprises the outer and inner membranes that are composed of the translocon protein complexes Toc and Tic (translocon at the outer/inner envelope membrane of chloroplasts), respectively. Tic55, a chloroplast Tic protein member, was shown to be not vital for functional protein import in Arabidopsis from previous … ts type to stringWebbCreate Professional Science Figures in Minutes. Explore Biorender. GraphPad Prism is the leading Scientific Graphing Software for data analysis and visualization -built for scientific research. Prism is a versatile statistical graphing program and graph plotting software that is used by scientists—not statisticians. No coding required. ts type templateWebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … ph levels for african cichlidsWebbIt's not cheap ($850 for a single user license) but it's a lot less than comparable suits (like Lasergene and Sequencher, each costs about $2000 for a single user license). I tried out Benchling but they didn't suit my needs and the subscription was essentially the same cost as a Geneious license. cymicro • 8 yr. ago. ts typewriting exam notification 2022WebbSnapGene will automatically check each day and alert you if an update is available that is valid for your license. The automated checking can be turned off in Preferences - see Set … ph levels for acids and baseshttp://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html ph levels for acidshttp://www.coolaber.com/usr/2160/File/F/F4671.pdf ts type typeof