NettetWash the filter with 5 mL of PEB buffer. Discard the filter and centrifuge cell suspension at 300×g for 10 minutes at room temperature. Aspirate supernatant completely. … NettetWhole blood samples stained with antibodies for cell-surface markers can be analyzed directly on a flow cytometer. To facilitate this analysis, red blood cells (RBCs) in the …
Voided stain on paper method for analysis of mouse urination
Nettet2. jan. 2024 · Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3’-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell … Nettet1. jan. 2008 · Voiding behavior of the mice was analyzed by placing the animal above the same filter paper and recording voided time and area over 2 hr. The usefulness of the VSOP method was tested in analysis of the voiding behavior of five female 7-week-old ddY mice treated with cyclophosphamide (CPM, 150 mg/kg, intraperitoneally) and five … thermos original
Whole Blood Staining Protocol for Flow Cytometry Analysis
Nettet13. jul. 2024 · The aim of this study was to evaluate the cytotoxicity of a Thai herb by comparing MTT and SRB assay results. Mouse fibroblast (L929) cells were exposed to 0.01, 0.1, 0.25, and 0.5% (w/v) ... Vajrabhaya et al. compared the SRB staining assay against 51 Cr-release in cytotoxicity tests of six endodontic sealers. In their study, ... Nettet9. des. 2024 · The voiding spot assay (VSA) on filter paper is an increasingly popular method for studying lower urinary tract physiology in mice. However, ... Voided stain on paper method for analysis of mouse urination. Neurourol Urodyn 27: 548–552, 2008. doi: 10.1002/nau.20552. Nettet18. des. 2024 · This suggests an overall lower catalytic activity in the filter paper assay, which may be due to inactivation or loss of one or a combination of the reaction components. Similarly, the LOD and LOQ are lower in bulk measurements compared to measurements in the detector, although they remain in both cases in the sub … thermos original flask