Hitrap desalting说明书
WebThe effect of different sample volumes on the HiTrap Desalting column using tubing loops for sample injection. Column: HiTrap Desalting Sample: 2 mg/ml bovine serum albumin in 50 mM sodium phosphate buffer, 0.5 M sodium chloride, pH 7.0 Sample volume: 0.5 ml, 1.1 ml, 2.1 ml Eluent: 50 mM sodium phosphate buffer, 0.15 M sodium chloride, pH 7.0 Web11 lug 2010 · Keep flowrate range1–10 ml/min. Collect fractions. approximately10 ml buffer before applying nextsample. Collect fractions. Note: sampleinjection tubingsample loops …
Hitrap desalting说明书
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Webhitrap desalting脱盐柱用法 使用凝胶过滤介质SephadexG10,G15,G25,G50等去除小分子,效率高,处理量可达床体积30%。 WebHiPrep 26/10 Desalting 1 (53 ml) 17-5 087-01 HiTrap Desalting 5 × 5 ml 17-1408-01 PD-10 Desalting columns 30 17-0 851-01 Accessories Tubing connectors for connection to ÄKTA design: Union 1/16 ″ female/M6 male* 6 18-1 112-57 Union M6 female/1/16 ″ male 5 18-3858-01 Domed nut 4 18-2 450-01 Technical literature: Gel Filtration Handbook
Web[PDF] Data File: HiTrap Desalting, HiPrep 26/10 Desalting EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar … WebThe effect of different sample volumes on the HiTrap Desalting column using tubing loops for sample injection. Column: HiTrap Desalting Sample: 2 mg/ml bovine serum albumin …
WebDesalting and buffer exchange are methods to separate soluble macromolecules from smaller molecules (desalting) or replace the buffer system used for another one suitable for a downstream application (buffer exchange). These methods are based on gel filtration chromatography, also called molecular sieve chromatography, which is a form of size … WebHiTrap ® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different …
Web11 HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 Desalting columns if necessary (Table 5). Note: The reuse of HiTrap rProtein A FF depends on the nature of the sample and should only be performed with identical monoclonals to prevent cross-contamination. 10 …
WebInstructions for Use HisTrap™ HP is a ready to use HiTrap™ column, prepacked with precharged Ni Sepharose™ High Performance . This prepacked column is ideal for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). how to use sfc and dismWebHiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess resin for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP). Change Product Product Support FAQ Troubleshooting Accessories FAQ organochlorine meaningWebCytiva HiTrap™ Desalting Prepacked Columns For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances. Promotions are available 5 Cytiva PD SpinTrap™ G-25 Desalting Columns how to use sfmWebHiPrep 26/10 Desalting is a prepacked, ready-to-use column for group separation between high and low molecular weight substances. Applications include buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants, or removal of reagents to terminate a reaction. Read more Product specifications organochlorines and human health effectsWebFor desalting and buffer exchange of protein extracts (M r > 5000). HiTrap Desalting 17-1408-01 0.25–1.5 ml 1.0–2.0 ml Prepacked with Sephadex G-25 Superfine. Requires a syringe or pump to run. PD-10 Desalting 17-0851-01 1.0–2.5 ml 1 1.75–2.5 ml 2 3.5 ml 1 Up to 2.5 ml 2 Prepacked with Sephadex G-25 Medium. Runs by gravity flow or ... how to use sfml in visual studio codeWebHiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction. Application how to use sf2 in audacityWeb17-1408-01 HiTrap Desalting 5 × 5 ml 11-0003-29 HiTrap Desalting 100 × 5 ml* * Special pack delivered on specific customer order. Connectorkit Connectors supplied Usage No. supplied 1/16” male/luer female Connection of syringe to top of HiTrap column 1 Tubing connector Connection of tubing (e.g. Peristaltic flangeless/M6 female Pump P1) to ... organochlorines mode of action