WebNov 2, 2024 · As such, researchers developed new tools called FLEx/DIO vectors where the transgene is floxed by two pairs of recombination sites and the open reading frame is … WebDouble Floxed Inverted ORF Control Karl Deisseroth Lab: Double Floxed Inverted ORF Control Unpublished Plasmids from Article. ID Plasmid ... Purpose ; 27056: pAAV-Ef1a-DIO EYFP: Loading... Recombinant Antibodies from Article. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog ...
Addgene: Double Floxed Inverted ORF Control
WebMay 12, 2024 · Under the control of the Double-floxed Inverted Orientation (DIO) Cre-On system, AAV-DIO-TK-GFP expresses TK and GFP only in the presence of Cre recombinase (Fig. 3a), which assists H129-ΔTK-tdT monosynaptic transmission specifically from the Cre expressing neurons . WebOct 14, 2024 · Inverted APEX-NES (nuclear exporting sequence) is flanked by lox sites in a double floxed inverted orientation (DIO). (B) CRISPR/Cas9 strategy for mouse line generation. Enhanced specificity Cas9 (ESPCas9), targeting vector, and crRNA::tracrRNA duplex were used in pronuclear injection to generate founders. Agarose gel … gta 3 asuka missions
Virginia Commonwealth University VCU Scholars Compass
WebThis double-floxed inverted (DIO) PPO is in an inverted orientation and flanked by non-homologous recombination sites (LoxP and Lox2272). Co-expression with Cre recombinase flips the construct and excises these sites, resulting in proper orientation of the PPO-Venus transgene, with expression driven by the upstream CAG promoter. WebJun 8, 2014 · Diversifying the single recombinase–dependent AAVs. We first sought to expand the 'double-floxed' inverted open reading frame (DIO) Cre-dependent expression system 5,6,7,8 by developing ... WebDec 21, 2024 · An alternative strategy would be to use mouse models that express Cre in a defined cell type. The transfer vector would then include the promoter and a double-floxed inverted orientation (DIO) sequence which is inverted upon Cre activation . The tamoxifen-inducible Cx3cr1 CreERT2 mouse model has frequently been used to target … pikkis hukkanen