WebThe following procedure for obtaining insect DNA is similar to a plant DNA extraction procedure (Saghai-Maroof et al. 1984) and relies on a nonionic detergent, hexadecyltrimethylammonium bromide (CTAB) to lyse cells. The resulting DNA is suitable for use as a template in polymerase chain reactions to generate RAPD markers or for … WebMar 18, 2024 · The CTAB DNA extraction method is cheap, effective and applicable for a wide range of applications including DNA barcoding, shotgun sequencing and long-read sequencing. The protocol utilizes organic solvents and the harmful nature of some of the solvents, combined with the relatively long time to complete the protocol, can therefore …
Modified low-salt CTAB extraction of high-quality DNA from
WebDec 4, 2014 · We extracted eDNA from PCTE filters using our modified CTAB protocol ( Protocol S1 ), including one extraction negative control with every batch of extractions and pGEM-3Z plasmid in the CTAB buffer at 0.02 ng•µL −1 … Webwhere, C 0 is the spiked DNA concentration and C f is the DNA concentration in the DES-rich phase after extraction. V 0 is the volume of the DNA sample and V f is the volume of the DES-rich phase.. Sample preparation A human whole blood sample (200 μL) was mixed with proteinase K (20 μL, 100 mg mL −1) and Triton X-100 (20 μL) and the mixture was … phil montgomery director
Protocol for Genomic DNA extraction with CTAB — …
WebThere are many DNA extraction protocols that vary from simple and quick ones that yield low-quality DNA but good enough for routine analyses to the laborious and time … WebJan 3, 2024 · The DNA from the grains of two maize hybrids, M10 and M321, was extracted using extraction methods DNeasy Qiagen Plant Mini Kit, CTAB-method (with/without 1% PVP) and modified Mericon extraction. Genes coding for 45S ribosomal RNA are organized in tandem arrays of up to several thousand copies and contain codes for 18S, 5.8S and … Web2. Transfer CTAB/plant extract mixture to a microfuge tube. 3. Incubate the CTAB/plant extract mixture for about 15 min at 55° C in a recirculating water bath. 4. After incubation, … phil montgomery linkedin