Adipocyte rna-seq

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August undefined, 2024

WebFeb 8, 2024 · RNA-seq was used to determine genes regulated by EGCG and identify the key differences between the two functionally distinct adipose tissues. WebApr 12, 2024 · IMF deposition primarily occurs through the increase in the numbers of preadipocytes (cell proliferation) and adipocyte size (cell enlargement) . Intramuscular preadipocytes ... and stored in a −80 °C refrigerator for later RNA-seq, and part of the samples were placed in an incubator in an ice pack and then quickly sent to the …

Full article: RNA-Seq and lipidomics reveal different adipogenic ...

WebNov 8, 2024 · The snRNA-seq data of digested adipocytes from inguinal WAT reveals a complex subpopulation of mature adipocytes with distinct genetic signatures [ 35 ]. Recently, snRNA-seq analysis of the WAT identified a rare subpopulation of adipocytes in mice that increase in abundance at higher temperatures. WebApr 19, 2024 · RNA sequencing (RNA-Seq) was conducted to explore the chicken transcriptome from the thigh muscle tissue of 6 Huangshan Black Chickens with 3 extremely high and low phenotypic values for percentage of polyunsaturated fatty acids (PUFAs). rockdale healthcare

Cancers Free Full-Text Inhibition of LIFR Blocks Adiposity …

WebDec 23, 2024 · Recent studies using single-cell RNA sequencing (scRNA-seq) in adipose tissue from mouse models have identified a subset of adipocyte progenitors that … WebFeb 3, 2024 · RNA-Seq analysis of the intramuscular adipocytes during adipogenesis To reveal the role of lncRNAs in the intramuscular adipogenesis, high-throughput … WebEndometrial cancer (EC) is the fourth most common cancer in women, and half of the endometrioid EC (EEC) cases are attributable to obesity. However, the underlying mechanism(s) of obesity-driven EEC remain(s) unclear. In this study, we examined whether LIF signaling plays a role in the obesity-driven progression of EEC. RNA-seq analysis of … ota in thailand

snRNA-seq reveals a subpopulation of adipocytes that regulates …

Deconstructing cold-induced brown adipocyte neogenesis in mice

WebNational Center for Biotechnology Information WebSep 16, 2024 · The transcriptome data was generated from the different stages of the 3T3-L1 adipocytes using RNA-seq technology. Table 2 briefly summarizes the information of … rockdale gymnastics hilton head scWebIn addition, adipose tissue functions as a signalling hub that regulates systemic metabolism through paracrine and endocrine signals 4. Here we use single-nucleus RNA-sequencing (snRNA-seq) analysis in mice and humans to characterize adipocyte heterogeneity. rockdale hardware

"WebNov 8, 2024 · Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their … " - Adipocyte rna-seq

Adipocyte rna-seq

Profiling mouse brown and white adipocytes to identify …

WebMar 7, 2024 · Results: In this study, we found that brown adipose tissue (BAT) existed within the perirenal fat at 1 day after birth (D1) and transferred into white adipose tissue (WAT) … WebMar 25, 2024 · However, single-nuclei RNA sequencing (sn-RNAseq) and spatial transcriptomics have created an avenue to explore the complexity of adipocyte status and function in vivo. While current knowledge keeps expanding, it is now clear that distinct adipocyte populations exist within WAT depots.

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WebMar 16, 2024 · As expected, adipocytes were found only in the sNuc-seq dataset. The sNuc-seq data were also enriched for vascular cells and macrophages, probably because collagenase digestion did not fully... WebApr 30, 2024 · a Single-cell RNA seq was performed on differentiating preadipocytes beginning at 80% confluency and differentiated for 7 days. PAGODA was used to …

WebMay 20, 2024 · In conclusions, this RNS-Seq study finds that peripheral subcutaneous adipose tissue among Asian Indians show pathology characterized by altered lipid, … WebDec 3, 2024 · Transcriptome-wide m 6 A-Seq Reveals Global m 6 A Modification Patterns During Yak Adipocyte Differentiation. The yak adipocyte and preadipocyte of three biological replicates were used for transcriptome-wide m 6 A-sequencing (m 6 A-seq) and RNA-sequencing (RNA-seq) assays. In total, 12 libraries were sequenced, comprising …

WebWe analyzed adipose gene expression using RNA sequencing in the fenofibrate and omega-3 fatty acid modulation of endotoxemia (FFAME) trial of healthy individuals at three … WebSep 9, 2016 · Adipogenesis of primary brown preadipocytes In order to get a global view of gene expression during adipogenesis of brown preadipocytes, RNA-Seq was performed to identify the genes involved and their function. RNA was isolated from primary cultured brown preadipocytes (D0) and differentiated adipocytes (D7).

WebAug 8, 2024 · RNA extraction and RNA-seq analysis were performed by NovoGene (Beijing, China), the sequencing platform was illumina novaseq 6000, and the read length was 150bp. The sampling time points were listed in Figure 1. FeatureCounts (1.5.0-p3) is used to calculate the reads mapped to each gene.

WebHere we use single-nucleus RNA-sequencing (snRNA-seq) analysis in mice and humans to characterize adipocyte heterogeneity. We identify a rare subpopulation of adipocytes in … rockdale halal meatWebNov 27, 2024 · Single‐cell RNA sequencing and lipidomics reveal cell and lipid dynamics of fat infiltration in skeletal muscle - PMC Back to Top Skip to main content An official website of the United States government Here's how you know The .gov means it’s official. Federal government websites often end in .gov or .mil. ota_iphone4.1.ipswWebMar 8, 2024 · We performed RNA-Seq on adipocytes isolated from three different adipose depots (BAT, iWAT, and eWAT) in HFD and ND. More than 200 million paired-end reads … rockdale habitat for humanityWebHere, it investigated transcriptional profiling of adipogenesis from human mesenchymal stem cells (hMSCs) by RNA-Seq transcriptome technique. Oil Red O staining assays were … otaio shipWebSep 17, 2024 · Here, we provide an optimized protocol for nuclei isolation from mouse adipose tissues suitable for single-nucleus RNA sequencing. This allows for transcriptomic profiling of the entire adipose tissue at single-cell resolution. For complete details on the use of this protocol, please refer to Sárvári et al., 2024. rockdale hairWebDec 21, 2024 · Single nucleus sequencing (snRNAseq) is a modified version of scRNAseq, wherein a single nucleus is sequenced for actively transcribed RNA instead of a single cell. This technique is especially effective on tissues that cannot be dissociated into intact single cells, such as adipocytes. rockdale health clinicWebNov 7, 2024 · RNA was prepared from eight different cell WAT cell types (ASCs, total adipose tissue macrophages (ATMs), M1 ATMs, M2 ATMs, total T cells, CD4 + T cells, CD8 + T cells, and mature adipocytes). Ten nanograms of RNA was amplified using four cycles and loaded onto Clariom™D microarray chips. For details see Additional file 1: … ota ipad2 2 download